Summary
Type: SPE
Application #: 305170
Catalog#: 730127 - SPE Cartridges, Chromabond, Polyamide 6, 6ml, PP, 1000mg, 30/pk
Detection:
Conditions: Sample aspiration: The prepared sample were passed through the column by vacuum
Washing: With 20 ml 1 % acetic acid in water, sugars and proteins were removed.
Elution: Flavonoids were eluted with 80 ml methanol
Evaporation under reduced pressure at 35 °C
Reconstitution in methanol/water (20:80, v/v) to the orginal volume of 10 ml.
Authors: Papp, A.; Winnewisser, W.; Geiger, E.
Source: J. Institute Brewing, Vol 107, No. 1, 2001, 55-60